1. What is HRIPT/RIPT?

RIPT: Repeated Insult Patch Test (RIPT), which is the most rigorous research at present. There is no potential irritation or allergy after using products or raw materials. Nine groups of induced patches are used, and a period of rest This was followed by a set of challenge tests.
HRIPT: Human Repeated Insult Patch Test (HRIPT)

2. Test process
▨ After the sample arrives at the laboratory, the test substance will be given an independent laboratory test number, and the batch number, sample specification, sponsor, receipt date and test request will be recorded and verified in the laboratory log.
▨Volunteer screening: According to the opinion of the research and development staff, the tester must not have any systemic disease or skin history, otherwise it will hinder the test results or increase the risk of adverse reactions in the body. If the skin color is suitable for erythema test observation, any skin Nature and any race can participate, and a research sample of 53 people was finally screened.
▨The test is divided into an induction period and a challenge period. Through repeated tests, it is finally determined whether the subjects have erythema or other adverse stimulation reactions.
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2. What is BCOP?

Amazon US station for eye shadow, liquid eyeliner, magnetic eyelashes, false eyelashes and other products, need to provide BCOP certification. Because these products may cause some irritation to the eyes.
What is BCOP certification?
The bovine corneal opacity and permeability test (BCOP ) is an in vitro eye irritation test developed by Gautheron et al. (1992). This test evaluates the eye irritation potential of the test article using fresh bovine cornea obtained from a slaughterhouse. The type of damage caused by the exposure of the test article was quantitatively detected by the changes in the turbidity of the cornea and the permeability to fluorescein.
The BCOP test can be used to study the mechanisms that lead to injury. Protein degeneration or stromal swelling can lead to corneal opacity, while impaired corneal barrier function and cell membrane junctions between corneal epithelial cells can manifest as changes in corneal permeability.
In addition, histological endpoints can be supplemented to assess corneal swelling, dehydration, or morphological changes. Histological evaluation is mainly to evaluate the type of damage observed and the depth of damage to the cornea.
Methods The chicken embryo allantoic membrane vascular test (CAMVA), the bovine corneal opacity permeability test (BCOP) and the Draize rabbit eye test were used to test 60 kinds of cosmetics, and to predict the classification of eye irritation.
Results 41 non-irritating samples and 18 irritating samples could be distinguished by CAMVA method;
35 non-irritant, 21 mild-moderate irritant and 4 severe irritant samples were predicted by BCOP method;
The combined CAMVA-BCOP method can significantly improve the discrimination ability of stimulus degree, and the consistency with the in vivo experimental classification is 98.3%.
Conclusion The integrated test strategy of combined CAMVA-BCOP can be used for the eye irritation evaluation of cosmetic products, and its prediction range can cover non-irritant to severe irritation.

Cosmetics HRIPT / RIPT+COA certificate processing process:

1. Fill in the cosmetic HRIPT/RIPT application form + COA application form

2. Sign the contract

3. Send samples

4. Arrange payment

5. Issue the draft report

6. Issue official documents